Journal: American Journal of Translational Research

Article Title: Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation

doi:

Figure Lengend Snippet: Primers used for qRT-PCR

Article Snippet: Primary anti-rat CD163 antibody (NBP2-39099, Novus Biologicals Europe) was used at a dilution of 1:100; primary anti-rat MHC-II antibody (ab23990, Abcam) was used at a dilution of 1:300, whereas the PBS was used as a negative control.

Techniques: Sequencing

Journal: American Journal of Translational Research

Article Title: Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation

doi:

Figure Lengend Snippet: The infiltration of the CD163-positive cells increased in the tolerant liver grafts. A. Representative images of HE staining of liver allografts in the tolerance and the AR group 7 days following transplantation with original magnifications of ×100. Characteristics like portal inflammatory cell infiltration, bile duct damage and endothelial inflammation significantly reduced in the tolerance group. Scale bar in right lower corner represents 100 µm. B. Liver functions were assessed on day 7 after transplantation. Both ALT and AST were significantly lowered in tolerance group. C, D. ELISA was used to detect serum IL-10 and TGF-β1 levels of the recipients in both groups. Both anti-inflammatory cytokines were significantly increased in the tolerant recipients. E. Illustrating IHC microscopic finding for identification of CD163 positive cells (brown color) with original magnifications of ×400. Scale bar in right lower corner represents 25 µm. F. Analytical results of the numbers of CD163 positive cells. The numbers of CD163 positive cells in the AR group were less than that in the tolerance group. All statistical analyses were performed by an unpaired t-test. Data are presented as the mean ± SD. (n = 5, *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Primary anti-rat CD163 antibody (NBP2-39099, Novus Biologicals Europe) was used at a dilution of 1:100; primary anti-rat MHC-II antibody (ab23990, Abcam) was used at a dilution of 1:300, whereas the PBS was used as a negative control.

Techniques: Staining, Transplantation Assay, Enzyme-linked Immunosorbent Assay

Journal: American Journal of Translational Research

Article Title: Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation

doi:

Figure Lengend Snippet: M2c BMDMs were successfully induced in vitro. A. The bone marrow derived cells were examined by immunofluorescence staining with anti-CD68 antibody after being stimulated by MCSF for 7 days. Nearly all cells expressed CD68, the specific rat macrophage marker (Magnification, 200). Scale bars in right lower corner represents 50 µm. These cells were then stimulated by PBS or dexamethasone for 24 h for M0 or M2c polarization. B. Representative images of the M0 and the M2c with original magnifications of ×400. Scale bar in right lower corner represents 25 µm. C. M2c polarization markers expression determined by qRT-PCR. The expression levels of CD163, IL-10, TGF-β1 in the M2c macrophages were significantly higher than those of the M0 macrophages. The statistical analyses were performed by an unpaired t-test. Data are presented as the mean ± SD. (n = 3, **P < 0.01).

Article Snippet: Primary anti-rat CD163 antibody (NBP2-39099, Novus Biologicals Europe) was used at a dilution of 1:100; primary anti-rat MHC-II antibody (ab23990, Abcam) was used at a dilution of 1:300, whereas the PBS was used as a negative control.

Techniques: In Vitro, Derivative Assay, Immunofluorescence, Staining, Marker, Expressing, Quantitative RT-PCR

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: IL-13–regulated Macrophage Polarization during Granuloma Formation in an In Vitro Human Sarcoidosis Model

doi: 10.1165/rcmb.2018-0053oc

Figure Lengend Snippet: Figure 5: Immunofluorescence imaging of granuloma-like structures demonstrates persistent macrophage CD163 expression on sarcoidosis PBMCs following PPD stimulation. Representative photomicrographs of immunostained PBMCs in granuloma-like structures 7 days after bead treatment using

Article Snippet: Copyright © 2018 by the American Thoracic Society Alternatively, following washing and blocking, some coverslips were incubated with 0.5 μg/ml CD163 [unlabeled, rabbit, anti-human CD163 mAb (K20-T, IgG) from Novus Biologicals, LLC (Littleton, CO)] or the matching isotype control antibody [Abcam, (Cambridge, MA)] at room temperature for 1 hour.

Techniques: Immunofluorescence, Imaging, Expressing

Journal: Toxicologic Pathology

Article Title: Biocompatible Solutions: Evaluating the Safety of Repeated Intra-Articular Injections of pMPCylated Liposomes for Knee Osteoarthritis Therapy in Rat Models

doi: 10.1177/01926233241271400

Figure Lengend Snippet: Antibodies used for the immunofluorescence study.

Article Snippet: Novus (CD163) , NBP3-07981 (CD163).

Techniques: Immunofluorescence, Marker